Step Pathway KO FRH46_2 FRH46_3 FRH46_4 FRH46_5 FRH46_6 FRH47_2 FRH47_3 FRH47_4 FRH47_5 FRH47_6 FRH48_2 FRH48_3 FRH48_4 FRH48_5 FRH48_6 FRH49_3 FRH49_4 FRH49_5 FRH49_6 FRH50_3 FRH50_4 FRH50_5 FRH50_6 FRH51_2 FRH51_3 FRH51_4 FRH51_5 FRH51_6 FRH52_3 FRH52_4 FRH52_5 FRH52_6 FRH53_3 FRH53_4 FRH53_5 FRH53_6 FRH54_4 FRH54_5 FRH54_6 FRH55_2 FRH55_4 FRH55_5 FRH55_6 FRH56_2 FRH56_4 FRH56_5 FRH56_6 FRH57_3 FRH57_4 FRH57_5 FRH57_6 Acetyl-Co-A to Pyruvate Reductive citrate cycle K00169 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Acetyl-Co-A to Pyruvate Reductive citrate cycle K03737 4 1 2 3 7 0 3 4 4 7 2 1 0 2 1 6 7 11 11 5 3 10 11 4 2 3 4 4 1 1 0 1 2 1 2 3 2 1 0 0 0 2 1 0 0 0 0 1 0 1 1 Pyruvate to Phosphoenolpyruvate Reductive citrate cycle K01006 14 6 7 11 19 3 24 40 41 41 8 5 4 9 30 21 16 28 28 20 41 75 57 7 6 6 10 9 9 11 12 19 12 18 45 43 10 2 3 3 4 11 29 6 12 0 3 24 19 38 46 Pyruvate to Phosphoenolpyruvate Reductive citrate cycle K01007 3 0 0 0 4 0 1 5 5 6 1 1 0 3 7 5 2 4 3 3 2 12 12 0 0 0 0 1 3 3 1 11 4 3 11 21 2 0 0 0 0 2 4 2 0 1 0 5 2 5 8 Pyruvate to Oxaloacetate Reductive citrate cycle K01958 4 2 2 5 5 1 9 11 13 11 2 2 3 4 11 5 2 6 7 7 16 26 15 0 1 2 5 5 4 7 8 7 4 10 19 13 6 1 1 2 4 6 12 2 8 0 1 15 12 16 21 Phosphoenol pyruvate to Oxaloacteate Reductive citrate cycle K01595 12 2 5 5 11 2 10 15 16 14 2 1 1 7 15 10 8 12 12 10 15 30 28 5 5 5 5 6 5 1 7 9 5 9 15 11 4 1 2 0 0 4 18 5 2 0 2 6 6 14 15 Oxaloacetate to S-Malate Reductive citrate cycle K00024 18 6 7 12 21 4 31 50 51 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 26 14 22 51 48 13 2 3 3 4 12 36 6 13 1 4 27 22 45 54 S-Malate to Fumarate Reductive citrate cycle K01676 9 0 1 4 9 2 11 19 20 16 0 2 2 6 13 9 8 9 7 9 10 20 21 3 4 5 6 6 4 6 7 8 6 7 15 10 6 1 3 0 0 5 19 3 6 0 2 5 5 11 14 Fumarate to Succinate (bidirectional) Reductive citrate cycle K00239 18 6 8 12 22 3 31 50 52 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 26 14 22 51 48 13 2 3 3 4 12 36 6 13 1 4 27 22 45 54 Fumarate to Succinate (bidirectional) Reductive citrate cycle K00244 2 0 1 1 3 1 0 2 2 2 0 0 0 1 1 1 1 2 1 3 1 4 5 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 1 0 0 0 4 0 0 0 0 1 1 2 2 Succinate to Succinyl-CoA Reductive citrate cycle K01902 18 6 8 12 22 4 31 50 52 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 45 54 Succinyl-CoA to 2-Oxoglutarate Reductive citrate cycle K00174 6 1 2 3 7 0 2 5 4 7 2 1 0 3 2 5 7 12 11 4 4 11 13 6 3 3 4 4 5 9 2 12 2 0 8 19 5 1 0 0 0 5 5 1 2 0 1 6 2 5 5 2-Oxoglutarate to Isocitrate Reductive citrate cycle K00031 18 6 8 12 22 4 31 50 52 47 8 5 5 15 38 22 17 29 28 22 44 82 64 8 9 9 13 13 10 11 17 18 13 22 47 36 12 2 3 3 4 11 37 6 13 1 4 23 21 42 51 Isocitrate to cis-Aconitate Reductive citrate cycle K01681 18 6 8 10 21 4 31 50 52 47 8 5 5 15 39 21 17 29 28 21 45 82 64 8 9 9 13 13 12 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 43 54 cis-Aconitate to Citrate Reductive citrate cycle K01681 18 6 8 10 21 4 31 50 52 47 8 5 5 15 39 21 17 29 28 21 45 82 64 8 9 9 13 13 12 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 43 54 Tetrahydofolate to 10-Formyl-THF Wood-Ljungdahl pathway K01938 7 4 6 8 13 3 21 32 35 29 3 1 3 5 24 13 9 15 14 14 32 56 35 1 3 3 5 6 8 13 12 20 8 14 37 37 7 0 2 3 4 7 23 2 8 0 1 22 18 33 41 10-Formyl-THF to 5,10-Methenylene-THF Wood-Ljungdahl pathway K01491 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 5,10-Methenyl-THF to 5,10-Methylene-THF Wood-Ljungdahl pathway K01491 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 18 5,10-Methylene-THF to 5-Methyl-THF Wood-Ljungdahl pathway K00297 18 6 8 12 22 4 31 49 51 46 8 5 5 15 39 22 17 29 28 22 45 81 63 8 9 9 13 13 10 10 16 18 13 22 47 34 12 2 3 3 4 11 36 6 13 1 4 23 21 42 51 Acetate to Acetyl phosphate (bidirectional) Phosphate acetyltransferase-acetate kinase pathway K00925 14 5 5 8 17 2 14 24 25 28 6 4 2 7 18 14 11 19 19 15 26 51 41 6 4 5 6 7 9 7 8 9 8 14 26 22 6 1 1 0 3 7 22 5 7 1 1 17 12 17 20 Acetyl phosphate to Acetyl-CoA (bidirectional Phosphate acetyltransferase-acetate kinase pathway K00625 6 1 3 4 4 1 3 4 4 6 3 2 2 4 4 3 3 7 6 4 7 17 13 2 1 3 5 5 2 0 4 5 2 7 7 3 4 2 2 0 3 4 6 4 3 0 2 6 5 6 6 Acetyl-CoA to Pyruvate Dicarboxylate-hydroxybutyrate cycle K00169 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pyruvate to Phosphoenol-pyruvate Dicarboxylate-hydroxybutyrate cycle K01007 3 0 0 0 4 0 1 5 5 6 1 1 0 3 7 5 2 4 3 3 2 12 12 0 0 0 0 1 3 3 1 11 4 3 11 21 2 0 0 0 0 2 4 2 0 1 0 5 2 5 8 Phosphoenol-pyruvate to Oxaloacetate Dicarboxylate-hydroxybutyrate cycle K01958 4 2 2 5 5 1 9 11 13 11 2 2 3 4 11 5 2 6 7 7 16 26 15 0 1 2 5 5 4 7 8 7 4 10 19 13 6 1 1 2 4 6 12 2 8 0 1 15 12 16 21 Oxaloacetate to S-Malate Dicarboxylate-hydroxybutyrate cycle K00024 18 6 8 12 21 4 31 50 51 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 26 14 22 51 48 13 2 3 3 4 12 36 6 13 1 4 27 22 45 54 S-Malate to Fumarate (bidirectional) Dicarboxylate-hydroxybutyrate cycle K01676 9 0 1 4 9 2 11 19 20 16 0 2 2 6 13 9 8 9 7 9 10 20 21 3 5 5 6 6 4 6 7 8 6 7 15 10 5 1 3 0 0 5 19 3 6 0 2 5 5 11 14 Fumarate to Succinate (bidirectional) Dicarboxylate-hydroxybutyrate cycle K00239 18 6 8 12 22 4 31 50 52 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 45 54 Fumarate to Succinate (bidirectional) Dicarboxylate-hydroxybutyrate cycle K00244 2 0 1 1 3 1 0 2 2 2 0 0 0 1 1 1 1 2 1 3 1 4 5 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0 1 0 0 0 4 0 0 0 0 1 1 2 2 Succinate to Succinyl-CoA Dicarboxylate-hydroxybutyrate cycle K01902 18 6 8 12 22 4 31 50 52 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 45 54 Acetoacetyl-CoA to Acetyl-CoA Dicarboxylate-hydroxybutyrate cycle K00626 18 6 7 11 21 4 30 50 52 46 8 5 5 15 39 21 16 25 25 22 45 82 64 7 8 9 12 12 11 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 45 54 Acetyl-CoA to Malonyl-CoA Hydroxypropionate-hydroxybutyrate cycle K02160 18 6 8 12 22 4 31 49 51 46 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 13 18 26 14 22 52 47 13 2 3 3 4 12 36 6 13 0 4 27 22 45 54 Malonyl-CoA to Malonate semialdehyde Hydroxypropionate-hydroxybutyrate cycle K14468 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 Malonate semialdehyde to 3-Hydroxypropanoate Hydroxypropionate-hydroxybutyrate cycle K14468 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3-Hydroxypropanoate to 3-Hydroxypropionyl-CoA Hydroxypropionate-hydroxybutyrate cycle K14469 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3-Hydroxpropionyl-CoA to Acryloyl-CoA Hydroxypropionate-hydroxybutyrate cycle K14469 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 Acruloyl-CoA to Propionyl-CoA Hydroxypropionate-hydroxybutyrate cycle K14469 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 Propionyl-CoA to (S)-Methylmalonyl-CoA Hydroxypropionate-hydroxybutyrate cycle K15052 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 (S)-Methylmalonyyl-CoA to R-Methylmalonyl-CoA Hydroxypropionate-hydroxybutyrate cycle K05606 12 5 8 12 18 3 22 34 36 34 5 3 4 8 27 17 14 24 23 20 39 65 47 7 6 6 10 10 10 13 13 21 8 15 40 40 11 2 3 2 4 10 27 5 12 0 4 23 18 37 43 R-Methylmalonyl-CoA to Succinyl-CoA Hydroxypropionate-hydroxybutyrate cycle K01847 10 4 7 11 16 3 22 34 36 34 3 2 4 6 26 16 13 22 20 18 37 59 41 5 5 4 8 8 8 9 12 11 8 15 35 25 9 1 3 3 4 8 26 5 12 0 4 18 17 33 40 Acetoacetyl-CoA to Acetyl-CoA Hydroxypropionate-hydroxybutyrate cycle K00626 18 6 7 11 21 4 30 50 52 46 8 5 5 15 39 21 16 25 25 22 45 82 64 7 8 9 12 12 11 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 45 54 Acetyl-CoA to Malonyl-CoA 3-Hydroxypropionate bi-cycle K08691 7 2 4 6 12 2 13 25 26 25 3 0 3 2 15 10 10 14 12 9 23 39 28 2 3 3 5 5 6 9 10 10 7 10 28 21 5 0 1 3 3 6 20 2 9 0 1 13 12 23 31 Malonyl-CoA to Malonate semialdehyde 3-Hydroxypropionate bi-cycle K14468 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 Malonate semialdehyde to 3-Hydroxypropanoate 3-Hydroxypropionate bi-cycle K14468 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3-Hydroxypropanoate to 3-Hydroxypropionyl-CoA 3-Hydroxypropionate bi-cycle K14469 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3-Hydroxpropionyl-CoA to Acryloyl-CoA 3-Hydroxypropionate bi-cycle K14469 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 Acruloyl-CoA to Propionyl-CoA 3-Hydroxypropionate bi-cycle K14469 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 Propionyl-CoA to (S)-Methylmalonyl-CoA 3-Hydroxypropionate bi-cycle K15052 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 (S)-Methylmalonyyl-CoA to R-Methylmalonyl-CoA 3-Hydroxypropionate bi-cycle K05606 12 5 8 12 18 3 22 34 36 34 5 3 4 8 27 17 14 24 23 20 39 65 47 7 6 6 10 10 10 13 13 21 8 15 40 40 11 2 3 3 4 10 27 5 12 0 4 23 18 37 43 R-Methylmalonyl-CoA to Succinyl-CoA 3-Hydroxypropionate bi-cycle K01847 10 4 7 11 16 3 22 34 36 34 3 2 4 6 26 16 13 22 20 18 37 59 41 5 5 4 8 8 8 9 12 11 7 15 35 25 9 1 3 3 4 8 26 5 12 0 4 18 17 33 40 Fumarate to Succinate (bidirectional) 3-Hydroxypropionate bi-cycle K00239 18 6 8 12 22 4 31 50 52 47 8 5 5 15 39 22 17 29 28 22 45 83 65 8 9 9 13 13 12 14 18 27 14 22 52 48 13 2 3 3 4 12 37 6 13 1 4 27 22 45 54 Fumarate to Succinate (bidirectional) 3-Hydroxypropionate bi-cycle K00244 2 0 1 2 3 1 0 2 2 2 0 0 0 1 1 1 1 1 1 3 1 4 5 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0 1 0 0 0 4 0 0 0 0 1 1 2 2 Fumarate to S-Malate (bidirectional) 3-Hydroxypropionate bi-cycle K01676 9 0 1 4 9 2 11 19 20 16 0 2 2 6 13 9 8 9 7 9 10 20 21 3 4 5 6 6 4 6 7 8 6 7 15 10 5 1 3 0 0 5 19 3 6 0 2 5 5 11 14 L-Malonyl-CoA to Acetyl-CoA 3-Hydroxypropionate bi-cycle K08691 7 2 4 6 12 2 13 25 26 25 3 0 3 2 15 10 10 14 12 9 23 39 28 2 3 3 5 5 6 9 10 10 7 10 28 21 5 0 1 3 3 6 20 2 9 0 1 13 12 23 31 Propionyl-CoA to beta-Methylmalyl-CoA 3-Hydroxypropionate bi-cycle K08691 7 2 4 6 12 2 13 25 26 25 3 0 3 2 15 10 10 14 12 9 23 39 28 2 3 3 5 5 6 9 10 10 7 10 28 21 5 0 1 3 3 6 20 2 9 0 1 13 12 23 31 beta-Methylmalyl-CoA to 2-Methylfumaryl-CoA 3-Hydroxypropionate bi-cycle K14449 8 2 4 7 12 2 14 26 27 24 3 0 3 2 15 11 10 14 12 10 24 38 28 2 3 3 5 5 5 9 10 10 7 10 28 21 5 0 1 3 3 6 20 1 9 0 1 13 12 23 31 2-Methylfumaryl-CoA to 3-Methylfumaryl-CoA 3-Hydroxypropionate bi-cycle K14470 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3-Methylfumaryl-CoA to 3S-Citramalyl-CoA 3-Hydroxypropionate bi-cycle K09709 4 1 1 2 1 1 3 3 3 2 0 1 1 1 4 1 0 1 2 1 1 5 3 0 1 0 2 1 1 0 1 2 0 1 1 2 2 1 1 1 1 2 3 2 2 0 2 1 2 3 5 3S-Citramalyl-CoA to Acetyl-CoA 3-Hydroxypropionate bi-cycle K08691 7 2 4 6 12 2 13 25 26 25 3 0 3 2 15 10 10 14 12 9 23 39 28 2 3 3 5 5 6 9 10 10 7 10 28 21 5 0 1 3 3 6 20 2 9 0 1 13 12 23 31