%0 Generic %A RAJAGOPAL, VIJAYARAGHAVAN %A Bass, Gregory %A Walker, Cameron %A Crossman, David %A Petzer, Amorita %A Hickey, Anthony %A Siekmann, Ivo %A Hoshijima, Masahiko %A Ellisman, Mark H. %A J. Crampin, Edmund %A Soeller, Christian %D 2015 %T Examination of the effects of heterogeneous organization of RyR clusters, myofibrils and mitochondria on Ca2+ release patterns in cardiomyocytes %U https://melbourne.figshare.com/articles/dataset/Examination_of_the_effects_of_heterogeneous_organization_of_RyR_clusters_myofibrils_and_mitochondria_on_Ca2_release_patterns_in_cardiomyocytes/2001492 %R 10.4225/49/55B622EBE2D4C %2 https://melbourne.figshare.com/ndownloader/files/3001998 %2 https://melbourne.figshare.com/ndownloader/files/3002016 %2 https://melbourne.figshare.com/ndownloader/files/3001989 %2 https://melbourne.figshare.com/ndownloader/files/3002001 %2 https://melbourne.figshare.com/ndownloader/files/3002010 %2 https://melbourne.figshare.com/ndownloader/files/3002007 %2 https://melbourne.figshare.com/ndownloader/files/3001992 %2 https://melbourne.figshare.com/ndownloader/files/3002013 %2 https://melbourne.figshare.com/ndownloader/files/3002004 %2 https://melbourne.figshare.com/ndownloader/files/3002022 %2 https://melbourne.figshare.com/ndownloader/files/3002031 %2 https://melbourne.figshare.com/ndownloader/files/3002037 %K cardiac %K Computational biology %K Biophysics %K Cell Biology %K Biological Engineering %K Computational Biology %K Systems Biology %K Cardiology %K Numerical Analysis %K Geometry %X This dataset contains the electron microscopy and confocal microscopy data that were used in a study published in PLoS Computational Biology (see reference below). The dataset contains:

cardiac_cell_cross_section.rec: The 3D reconstructed electron tomogram that was used to generate a 3D model of myofibrils and mitochondria.

Files containing .mod extension: Model files generated using IMOD containing the manual segmentation of myofibrils, mitochondria and sarcolemma in the 3D electron tomogram.

Sham11_phalloidin_2_transv_dec.tif: Deconvolved confocal stack of myofibrils (stained by phalloidin) in the samples that were used for RyR cluster distribution analysis and simulation in this study.

Sham11_RyR_2*_transv_dec.tif: Deconvolved confocal image stack of the RyR clusters in the samples that were used for RyR cluster distribution analysis and simulation in this study.

Sham11_phalloidin_RyR_plectin_undeconvolved.tif: The raw, un-deconvolved confocal image stack that was used in this study. Contains RyR, phalloidin and plectin stained images. 
%I The University of Melbourne